Cell Culture Utensils
Sigma Aldrich Fine Chemicals Biosciences PyrexR cloning cylinder O.
Clone a single cell or group of cells by surrounding them with this Pyrex glass open ended cloning cylinder. Dip the end of the cylinder into sterile silicone grease before pressing to the bottom of a culture dish to create an isolated well. Cylinders are autoclavable or can be dry heat sterilized.
Bellco Glass, Inc. Cloning Cylinder,10x10mm
Used to surround a single cell to create homogeneous culture of cells rather than mixed population. The flat end ensures tight sealing with surface of culture dish. MADE IN THE USA. Sizes: 10x10mm
Bellco Glass, Inc. Cloning Cylinder,6x8mm
Used to surround a single cell to create homogeneous culture of cells rather than mixed population. The flat end ensures tight sealing with surface of culture dish. MADE IN THE USA. Sizes: 6x8mm 125 per case
Chemglass Life Sciences Cloning Cylinders, 8 x 8mm, 125pcs/Pack
Cloning Cylinders, 8 x 8mm, 125pcs/Pack. Clone a group or single cell by surrounding them with these glass cylinders. Cylinders are sealed to the plate with sterile silicone high vacuum grease, such as Dow Corning 976v thereby forming an isolation well. Cylinders are autoclavable or can be dry heat sterilized. Supplied in packages of 125 pieces.
Chemglass Life Sciences Cloning Cylinders, 10 x 10mm, 125pcs/Pack
Cloning Cylinders, 10 x 10mm, 125pcs/Pack. Clone a group or single cell by surrounding them with these glass cylinders. Cylinders are sealed to the plate with sterile silicone high vacuum grease, such as Dow Corning 976v thereby forming an isolation well. Cylinders are autoclavable or can be dry heat sterilized. Supplied in packages of 125 pieces.
Sigma Aldrich Fine Chemicals Biosciences Cloning cylinders glass vo1EA
Individual colonies of transfected cells can be isolated and picked from a plate containing many clones. Isolated clones can be dissociated and passaged free from surrounding cells or pulsed with 50-100 uL growth medium which can be analyzed for secreted products. The sterile cloning cylinders are greased on one end to allow the cylinder to seal to the plate. Cells are then dissociated within the cylinder resuspeneded and transferred to a new vessel as a pure colony.These cloning cylinders are sterlized using electron-beam irradiation. The glass cylinders will display a brown tint after being sterlized at the appropriate dosage level.